Cloning and expression of lipase gene from Ralstonia eutropha in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Cloning and expression of lipase gene from Ralstonia eutropha in Escherichia coli
Background Lipases are enzymes belonging to the class of hydrolases, acting in the aqueous-organic interface demonstrating considerable levels of activity and stability in aqueous environments and non-aqueous [1]. Its biological function is to catalyze the hydrolysis of long chain triglycerides to form fatty acids and glycerol, in addition to performing esterification [2]. They have wide indust...
متن کاملCloning and Expression of Mannheimia haemolytica PlpE Gene in Escherichia coli and its Immunogenicity Assessment
Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...
متن کاملCloning and expression of Eimeria necatrix microneme5 gene in Escherichia coli
Background: Coccidiosis caused by Eimeria necatrix has the most economic impact onpoultry production. Micronemal proteins in Eimeria necatrix are thoughtto be critical ligands determining host cell specificity at the time ofinvasion. OBJECTIVES: Isolation and purification of Eimeria necatrix oocysts from Khuzestan province of Iran was performed. AcDNA encoding microneme 5 (EnMIC5...
متن کاملCloning and optimization of phytase enzyme gene expression in Escherichia coli
Introduction Phytase is an enzyme that has the ability to break down phytic acid into myoinositol and mineral phosphate, and widely uses as an additive in animal foods. The aim of this study was to achieve a high level of bacterial phytase expression in PET26b expression host. Materials and Methods To generate the recombinant phytase enzyme, the target gene was introduced into the expression ...
متن کاملCloning and evaluation of gene expression and purification of gene encoding recombinant protein containing binding subunit of coli surface antigens CS1 and CS2 from Enterotoxigenic Escherichia coli
Background & Objective: Enterotoxigenic Escherichia coli (ETEC) is a major causative agent of diarrhea. Enterotoxins and the colonization factors (CFs) are major virulence factors in ETEC infections. The bacterium binds to the intestinal epithelial cell surface through colonization factors and produces enterotoxins that cause excessive fluid and electrolyte secretion in the lumen of the intesti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2014
ISSN: 1753-6561
DOI: 10.1186/1753-6561-8-s4-p239